Determination of Sugar in Blood
نویسنده
چکیده
In recent numbers of this Journal (1, 2) two criticisms have appeared of the present writer’s latest method (3) for the determination of blood sugar. The one of these by West, Scharles, and Peterson (1) is based wholly upon theoretical objections to a procedure which they have apparently not employed for even a single det,ermination. Condemnation based on what may be the shortcomings of a method need not be taken too seriously. We shall therefore not discuss the points raised in their communication. The three detailed alternative procedures for blood sugar determinations suggested by them may explain this theoretical attack against the only method so far proposed which will give apparently accurate and minimal sugar values when applied directly to tungstic acid blood filtrates. The criticism of the present writer’s blood sugar method presented by Everett (2) appears at first glance to be a detailed and careful study of the procedures involved. Even a cursory examination of the actual data offered serves, however, to indicate that Everett’s examination of the method was superficial and that he did not present findings related to the method as commonly employed. The essential criticism raised by Everett is that the present writer’s method does not give true blood sugar values because of rapid and unequal fading in color of the unknown solution in comparison with the standard. To a considerable extent Everett’s criticism resembles that of West and collaborators referred to above in that Everett fails to report any figures obtained through proper use of the method which he criticizes. The basis for Everett’s criticism rests chiefly upon the results recorded in his Table III which is herewith reproduced. As a result of the
منابع مشابه
A simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde
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